Glucocorticoid-stimulated lung epithelial Na(+) transport is associated with regulated ENaC and sgk1 expression.

Glucocorticoid-stimulated Na transport in human lung epithelia is associated with regulated ENaC and sgk1 expression. H441 CELLS, A BRONCHIOLAR EPITHELIAL CELL WITH GLUCOCORTICOID-REGULATED NA TRANSPORT, EXPRESS CLASSIC ENAC CHANNELS

Alveolar Epithelial Ion and Fluid Transport Glucocorticoid-stimulated lung epithelial Na transport is associated with regulated ENaC and sgk1 expression

OMAR A. ITANI,1 SCOTT D. AUERBACH,1 RUSSELL F. HUSTED,1 KENNETH A. VOLK,1 SHANA AGELOFF,2 MARK A. KNEPPER,2 JOHN B. STOKES,1,3 AND CHRISTIE P. THOMAS1,3 1Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242; 3Veterans Affairs Medical Center, Iowa City, Iowa 52246; and 2Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute,...

cAMP-stimulated Na+ transport in H441 distal lung epithelial cells: role of PKA, phosphatidylinositol 3-kinase, and sgk1.

H441 cells, a bronchiolar epithelial cell line, develop a cAMP-regulated benzamil-sensitive Na+ transport pathway on permeable supports (Itani OA, Auerbach SD, Husted RF, Volk KA, Ageloff S, Knepper MA, Stokes JB, Thomas CP. Am J Physiol Lung Cell Mol Physiol 282: L631-L641, 2002). To understand the molecular basis for the stimulation of Na+ transport, we delineated the role of specific intrace...

Epithelial sodium channel regulated by differential composition of a signaling complex.

Hormonal control of transepithelial sodium (Na(+)) transport utilizes phosphatidylinositide 3'-kinase (PI3K) and Raf-MAPK/ERK kinase (MEK)-ERK-dependent signaling pathways, which impact numerous cell functions. How signals transmitted by these pathways are sorted and appropriately transmitted to alter Na(+) transport without altering other physiologic processes is not well understood. Here, we ...

Serum- and glucocorticoid-regulated kinase 1 regulates ubiquitin ligase neural precursor cell-expressed, developmentally down-regulated protein 4-2 by inducing interaction with 14-3-3.

Serum- and glucocorticoid-regulated kinase 1 (SGK1) is an aldosterone-regulated early response gene product that regulates the activity of several ion transport proteins, most notably that of the epithelial sodium channel (ENaC). Recent evidence has established that SGK1 phosphorylates and inhibits Nedd4-2 (neural precursor cell-expressed, developmentally down-regulated protein 4-2), a ubiquiti...